Bioisis

"PER2 PAS-AB DIMER"

Experimental SAS Curve

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Experimental Mass

65,900 Da

Experimental Details for BID:  PLCSWP
Experiment ID: 113
Collected at: BL 12.3.1
Contributors: Partch, CP ,  Sandate, CS
SAXS profile of the tandem PAS-A PAS-B dimer of mouse PER2. Samples were purified by size-exclusion chromatography prior to SAXS analysis.
Data were collected @ 1,3, and 5mg/ml and extrapolated to zero concentration using the method of Zimm.

Electron Pair Distribution

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       Dmax → 71 Å


Guinier Plot

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     Guinier Rg → 26.9 Å

Real Space Rg → 26.86 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


PDB Model fit to SAXS Data

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The red line is the calculated SAXS profile from a PDB model scaled to the experimental SAXS curve (cyan). The two curves agree with a Chi2 of 3.9.


DAMMIN/F Model

DAMMIN/F result was determined with the following:

Space GroupP2
NSD | RMSD0.8 
variance(NSD | RMSD)0.1 
Number of Models in Average10 

Additional Experimental Details
Title

PER2 PAS-AB DIMER

Description

SAXS profile of the tandem PAS-A PAS-B dimer of mouse PER2. Samples were purified by size-exclusion chromatography prior to SAXS analysis.

Publication

In preparation

Contributors

Partch, CP ,  Sandate, CS

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: PER2

Annotation: tandem PAS-A and PAS-B domains of mouse PERIOD2

GAMDPSYSME QVEGITSEYI VKNADMFAVA VSLVSGKILY ISNQVASIFH CKKDAFSDAK FVEFLAPHDV SVFHSYTTPY KLPPWSVCSG LDSFTQECME EKSFFCRVSV GKHHENEIRY QPFRMTPYLV KVQEQQGAES QLCCLLLAER VHSGYEAPRI PPEKRIFTTT HTPNCLFQAV DERAVPLLGY LPQDLIETPV LVQLHPSDRP LMLAIHKKIL QAGGQPFDYS PIRFRTRNGE YITLDTSWSS FINPWSRKIS FIIGRHKVRV GPLNEDVFAA PPCPEEKTPH PSVQELTEQI HRLLMQPVP
categoryamino acid composition(%)
HydrophobicI(5.8) V(7.8) L(8.1) M(2.3) A(5.5) G(4.2) P(8.7)
AromaticF(5.5) W(1.0) Y(3.6)
HydrophilicR(4.9) K(4.9) E(7.1) D(3.9) Q(5.2) N(2.3) H(3.9) S(8.1) T(4.9) C(2.6)