| "Splicing factor SF1, residues 1-255 bound to RNA" |
|
|
|
Experimental Mass
32,300 Da |
| Experimental Details for BID: 1SF1KX |
|---|
| Experiment ID: 46
In addition, this experiment links to experiment(s):
45 47
48
|
| Collected at: ALS BL 12.3.1 |
| Contributors:
Kielkopf, C.L.
,
Jenkins, J.L.
,
Gupta, A.
|
| |
| Human SF1 bound to branch point sequence RNA from AdML 3' splice site (5'-UACUUAUCC-3')
|
| Merge of short and long exposures collected at three different concentrations (3, 6, and 12 mg/mL)
|
|
|
|
|
|
|
Dmax → 105 Å
|
|
|
|
|
|
Guinier Rg → 29.4 Å
Real Space Rg → 30.3 Å
|
|
The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.
|
|
|
| |
|
|
|
The Kratky plot is used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape similar to that for the Electron Pair Distribution Plot.
|
|
|
| |
|
DAMMIN/F result was determined with the following:
| Space Group | P1 |
| NSD | RMSD | 0.68 |
| variance(NSD | RMSD) | 0.2 |
| Number of Models in Average | 10 |
|
|
|
| Additional Experimental Details |
|---|
| Title Splicing factor SF1, residues 1-255 bound to RNA |
|---|
| Description Human SF1 bound to branch point sequence RNA from AdML 3' splice site (5'-UACUUAUCC-3')
|
|---|
| Publication RNA Induces Conformational Changes in the SF1/U2AF(65) Splicing Factor Complex. Gupta A, Jenkins JL, Kielkopf CL. J Mol Biol. 2010 Dec 10. PMID: 21146534
|
| Contributors
Kielkopf, C.L.
,
Jenkins, J.L.
,
Gupta, A.
|
| Genomics and Proteomics
The experiment is
composed of 2 genes/ORFs
Abbreviated name: SF1
Annotation:
N-terminal U2AF65-binding domain. Central/C-terminal; RNA binding KH domain
|
GPLGSMATGA
NATPLDFPSK
KRKRSRWNQD
TMEQKTVIPG
MPTVIPPGLT
REQERAYIVQ
LQIEDLTRKL
RTGDLGIPPN
PEDRSPSPEP
IYNSEGKRLN
TREFRTRKKL
EEERHNLITE
MVALNPDFKP
PADYKPPATR
VSDKVMIPQD
EYPEINFVGL
LIGPRGNTLK
NIEKECNAKI
MIRGKGSVKE
GKVGRKDGQM
LPGEDEPLHA
LVTANTMENV
KKAVEQIRNI
LKQGIETPED
QNDLRKMQLR
ELARLNGTLR
|
|---|
|
| category | amino acid composition(%) |
|---|
| Hydrophobic | I(6.2) V(4.6) L(8.8) M(3.5) A(4.6) G(7.3) P(9.2) |
|---|
| Aromatic | F(1.5) W(0.4) Y(1.5) |
|---|
| Hydrophilic | R(8.5) K(8.5) E(9.2) D(5.0) Q(4.2) N(6.2) H(0.8) S(3.1) T(6.5) C(0.4) |
|---|
|
Abbreviated name: BPS
Annotation:
branch point sequence of AdML splice site
|
| category | Residue composition (%) |
|---|
| Nucleotides |
G (0.0) C (33.3) A (22.2) U (44.4) T (0.0) |
|---|
| Mass: 2,957 Da | Total residues: 9 |
|
|
|
