| "Splicing factor complex of SF1 residues 1-255, U2AF65 residues 148-375 and AdML RNA" |
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Experimental Mass
74,300 Da |
| Experimental Details for BID: 1SUFKX |
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| Experiment ID: 48
In addition, this experiment links to experiment(s):
46 45
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| Collected at: ALS BL 12.3.1 |
| Contributors:
Kielkopf, C.L.
,
Jenkins, J.L.
,
Gupta, A.
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| Human SF1 ULM-KH-QUA2 bound to U2AF65 RRM1-RRM2-UHM and AdML 3' splice site RNA (5'-UACUUAUCCUGUCCCUUUUUUUUCC-3') in a splicing factor/splice site complex.
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| Merge of short and long exposures collected at three different concentrations (2.8, 5.6, and 11.2 mg/mL)
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Dmax → 135 Å
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Guinier Rg → 38.0 Å
Real Space Rg → 38.1 Å
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The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.
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The Kratky plot is used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape similar to that for the Electron Pair Distribution Plot.
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DAMMIN/F result was determined with the following:
| Space Group | P1 |
| NSD | RMSD | 0.69 |
| variance(NSD | RMSD) | 0.03 |
| Number of Models in Average | 10 |
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| Additional Experimental Details |
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| Title Splicing factor complex of SF1 residues 1-255, U2AF65 residues 148-375 and AdML RNA |
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| Description Human SF1 ULM-KH-QUA2 bound to U2AF65 RRM1-RRM2-UHM and AdML 3' splice site RNA (5'-UACUUAUCCUGUCCCUUUUUUUUCC-3') in a splicing factor/splice site complex.
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| Publication RNA Induces Conformational Changes in the SF1/U2AF(65) Splicing Factor Complex. Gupta A, Jenkins JL, Kielkopf CL. J Mol Biol. 2010 Dec 10. PMID: 21146534
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| Contributors
Kielkopf, C.L.
,
Jenkins, J.L.
,
Gupta, A.
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| Genomics and Proteomics
The experiment is
composed of 3 genes/ORFs
Abbreviated name: SF1
Annotation:
N-terminal; ULM for binding U2AF65 Central; KH-QUA2 for binding RNA
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GPLGSMATGA
NATPLDFPSK
KRKRSRWNQD
TMEQKTVIPG
MPTVIPPGLT
REQERAYIVQ
LQIEDLTRKL
RTGDLGIPPN
PEDRSPSPEP
IYNSEGKRLN
TREFRTRKKL
EEERHNLITE
MVALNPDFKP
PADYKPPATR
VSDKVMIPQD
EYPEINFVGL
LIGPRGNTLK
NIEKECNAKI
MIRGKGSVKE
GKVGRKDGQM
LPGEDEPLHA
LVTANTMENV
KKAVEQIRNI
LKQGIETPED
QNDLRKMQLR
ELARLNGTLR
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| category | amino acid composition(%) |
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| Hydrophobic | I(6.2) V(4.6) L(8.8) M(3.5) A(4.6) G(7.3) P(9.2) |
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| Aromatic | F(1.5) W(0.4) Y(1.5) |
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| Hydrophilic | R(8.5) K(8.5) E(9.2) D(5.0) Q(4.2) N(6.2) H(0.8) S(3.1) T(6.5) C(0.4) |
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Abbreviated name: U2AF65R12U
Annotation:
N-terminal; RRM1 for binding RNA Central; RRM2 for binding RNA C-terminal; UHM for binding SF1
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GPLGSARRLY
VGNIPFGITE
EAMMDFFNAQ
MRLGGLTQAP
GNPVLAVQIN
QDKNFAFLEF
RSVDETTQAM
AFDGIIFQGQ
SLKIRRPHDY
QPLPGMSENP
SVYVPGVVST
VVPDSAHKLF
IGGLPNYLND
DQVKELLTSF
GPLKAFNLVK
DSATGLSKGY
AFCEYVDINV
TDQAIAGLNG
MQLGDKKLLV
QRASVGAKNA
TLVSPPSTIN
QTPVTLQVPG
LMSSQVQMGG
HPTEVLCLMN
MVLPEELLDD
EEYEEIVEDV
RDECSKYGLV
KSIEIPRPVD
GVEVPGCGKI
FVEFTSVFDC
QKAMQGLTGR
KFANRVVVTK
YCDPDSYHRR
DFW
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| category | amino acid composition(%) |
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| Hydrophobic | I(4.2) V(9.9) L(9.0) M(3.3) A(5.7) G(8.7) P(6.6) |
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| Aromatic | F(5.1) W(0.3) Y(3.0) |
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| Hydrophilic | R(3.9) K(4.8) E(5.7) D(6.3) Q(5.4) N(4.5) H(1.2) S(5.7) T(4.8) C(1.8) |
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Abbreviated name: AdML3SS
Annotation:
3' splice site of AdML, including branch point sequence and polypyrimidine tract
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UACUUAUCCU
GUCCCUUUUU
UUUCC
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| category | Residue composition (%) |
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| Nucleotides |
G (4.0) C (32.0) A (8.0) U (56.0) T (0.0) |
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| Mass: 7,891 Da | Total residues: 25 |
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