| "Splicing factor U2AF65, residues 148-375 bound to RNA" |
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Experimental Mass
41,300 Da |
| Experimental Details for BID: 1U2FKX |
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| Experiment ID: 49
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| Collected at: ALS BL 12.3.1 |
| Contributors:
Kielkopf, C.L.
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Jenkins, J.L.
,
Gupta, A.
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| Human U2AF65 RRM1-RRM2-UHM bound to polypyrimidine tract RNA from AdML 3' splice site (5'-UCCCUUUUUUUUCC-3').
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| Merge of short and long exposures collected at three different concentrations (1.8, 3.6, 7.2, and 9 mg/mL)
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Dmax → 115 Å
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Guinier Rg → 32.1 Å
Real Space Rg → 33.8 Å
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The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.
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The Kratky plot is used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape similar to that for the Electron Pair Distribution Plot.
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DAMMIN/F result was determined with the following:
| Space Group | P1 |
| NSD | RMSD | 0.55 |
| variance(NSD | RMSD) | 0.01 |
| Number of Models in Average | 10 |
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| Additional Experimental Details |
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| Title Splicing factor U2AF65, residues 148-375 bound to RNA |
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| Description Human U2AF65 RRM1-RRM2-UHM bound to polypyrimidine tract RNA from AdML 3' splice site (5'-UCCCUUUUUUUUCC-3').
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| Publication RNA Induces Conformational Changes in the SF1/U2AF(65) Splicing Factor Complex. Gupta A, Jenkins JL, Kielkopf CL. J Mol Biol. 2010 Dec 10. PMID: 21146534
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| Contributors
Kielkopf, C.L.
,
Jenkins, J.L.
,
Gupta, A.
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| Genomics and Proteomics
The experiment is
composed of 2 genes/ORFs
Abbreviated name: U2AF65R12U
Annotation:
N-terminal; RRM1 (RNA binding motif) Central; RRM2 (RNA binding motif) C-terminal: UHM (SF1 interaction motif)
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GPLGSARRLY
VGNIPFGITE
EAMMDFFNAQ
MRLGGLTQAP
GNPVLAVQIN
QDKNFAFLEF
RSVDETTQAM
AFDGIIFQGQ
SLKIRRPHDY
QPLPGMSENP
SVYVPGVVST
VVPDSAHKLF
IGGLPNYLND
DQVKELLTSF
GPLKAFNLVK
DSATGLSKGY
AFCEYVDINV
TDQAIAGLNG
MQLGDKKLLV
QRASVGAKNA
TLVSPPSTIN
QTPVTLQVPG
LMSSQVQMGG
HPTEVLCLMN
MVLPEELLDD
EEYEEIVEDV
RDECSKYGLV
KSIEIPRPVD
GVEVPGCGKI
FVEFTSVFDC
QKAMQGLTGR
KFANRVVVTK
YCDPDSYHRR
DFW
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| category | amino acid composition(%) |
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| Hydrophobic | I(4.2) V(9.9) L(9.0) M(3.3) A(5.7) G(8.7) P(6.6) |
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| Aromatic | F(5.1) W(0.3) Y(3.0) |
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| Hydrophilic | R(3.9) K(4.8) E(5.7) D(6.3) Q(5.4) N(4.5) H(1.2) S(5.7) T(4.8) C(1.8) |
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Abbreviated name: Py-tract
Annotation:
polypyrimidine tract from AdML splice site
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| category | Residue composition (%) |
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| Nucleotides |
G (0.0) C (35.7) A (0.0) U (64.3) T (0.0) |
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| Mass: 4,440 Da | Total residues: 14 |
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