Bioisis

"Lysozyme Standard Curve"

Experimental SAS Curve

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Experimental Mass

14,300 Da

Experimental Details for BID:  LYSOZP
Experiment ID: 62
Collected at: ALS BL12.3.1
Contributors:
SAXS profiles for lysozyme at the concentration of ~2.5 mg/ml was collected at 100mM. SAXS data was collected and analyzed as described previously (Hura, 2009 Nature Method).
Extrapolated to zero concentration from 4 different concentrations between 1 and 10 mg/mL

Electron Pair Distribution

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       Dmax → 49 Å


Guinier Plot

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     Guinier Rg → 15.6 Å

Real Space Rg → 15.2 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


PDB Model fit to SAXS Data

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The red line is the calculated SAXS profile from a PDB model scaled to the experimental SAXS curve (cyan). The two curves agree with a Chi2 of 1.9.


Additional Experimental Details
Title

Lysozyme Standard Curve

Description

SAXS profiles for lysozyme at the concentration of ~2.5 mg/ml was collected at 100mM. SAXS data was collected and analyzed as described previously (Hura, 2009 Nature Method).

Publication

Contributors

Genomics and Proteomics

The experiment is composed of a single gene/ORF

Abbreviated name: LYSOZ

Annotation: LYSOZYME from Galis Galis

KVFGRCELAA AMKRHGLDNY RGYSLGNWVC AAKFESNFNT QATNRNTDGS TDYGILQINS RWWCNDGRTP GSRNLCNIPC SALLSSDITA SVNCAKKIVS DGNGMNAWVA WRNRCKGTDV QAWIRGCRL
categoryamino acid composition(%)
HydrophobicI(4.7) V(4.7) L(6.2) M(1.6) A(9.3) G(9.3) P(1.6)
AromaticF(2.3) W(4.7) Y(2.3)
HydrophilicR(8.5) K(4.7) E(1.6) D(5.4) Q(2.3) N(10.9) H(0.8) S(7.8) T(5.4) C(6.2)