Bioisis

"Splicing factor complex of phosphorylated (P)SF1 residues 1-255 and U2AF65 residues 148-375"

Experimental SAS Curve

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Low_res_thumbnail

Experimental Mass

66,113 Da

Experimental Details for BID:  1PSUKP
Experiment ID: 72
Collected at: ALS BL 12.3.1
Contributors: Wang, W ,  Kielkopf, CL
Human SF1 ULM-KH-QUA2, which is phosphorylated on Ser80Ser82, bound to U2AF65 RRM1-RRM2-UHM in a splicing factor complex.
Data from the low and high resolution ranges of the respective 0.5, 1 and 6-s exposure at 2.5, 5 and 10mg/ml were scaled and merged.

Electron Pair Distribution

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       Dmax → 135 Å


Guinier Plot

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     Guinier Rg → 39.1 Å

Real Space Rg → 39.8 Å

The Guinier plot is used to estimate the radius of gyration, Rg, which is taken from the slope of a line observed at low scattering angles (typically in the range where q* Rg < 1.3). This should be in reasonable agreement with the real space Rg.


Kratky Plot

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The Kratky plot can be used to visually assess the degree of "unfoldedness" of a protein or RNA sample. The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape.


DAMMIN/F Model

DAMMIN/F result was determined with the following:

Space GroupP1
NSD | RMSD0.76 
variance(NSD | RMSD)0.02 
Number of Models in Average10 

Additional Experimental Details
Title

Splicing factor complex of phosphorylated (P)SF1 residues 1-255 and U2AF65 residues 148-375

Description

Human SF1 ULM-KH-QUA2, which is phosphorylated on Ser80Ser82, bound to U2AF65 RRM1-RRM2-UHM in a splicing factor complex.

Publication

To investigate the role of phosphorylation on SF1 Ser80 & Ser82 in the SF1/U2AF65 splicing factor complex

Contributors

Wang, W ,  Kielkopf, CL

Genomics and Proteomics

The experiment is composed of 2 genes/ORFs

Abbreviated name: SF1

Annotation: N-terminal; ULM for binding U2AF65 Central; KH-QUA2 for binding RNA; phosphorylated SPSP motif

GPLGSMATGA NATPLDFPSK KRKRSRWNQD TMEQKTVIPG MPTVIPPGLT REQERAYIVQ LQIEDLTRKL RTGDLGIPPN PEDR(SEP)P (SEP)PEPIY NSEGKRLNTR EFRTRKKLEE ERHNLITEMV ALNPDFKPPA DYKPPATRVS DKVMIPQDEY PEINFVGLLI GPRGNTLKNI EKECNAKIMI RGKGSVKEGK VGRKDGQMLP GEDEPLHALV TANTMENVKK AVEQIRNILK QGIETPEDQN DLRKMQLREL ARLNGTLR
categoryamino acid composition(%)
HydrophobicI(6.0) V(4.5) L(8.6) M(3.4) A(4.5) G(7.1) P(9.7)
AromaticF(1.5) W(0.4) Y(1.5)
HydrophilicR(8.2) K(8.2) E(9.7) D(4.9) Q(4.1) N(6.0) H(0.7) S(3.0) T(6.3) C(0.4)

Abbreviated name: U2AF65R12U

Annotation: N-terminal; RRM1 for binding RNA Central; RRM2 for binding RNA C-terminal; UHM for binding SF1

GPLGSARRLY VGNIPFGITE EAMMDFFNAQ MRLGGLTQAP GNPVLAVQIN QDKNFAFLEF RSVDETTQAM AFDGIIFQGQ SLKIRRPHDY QPLPGMSENP SVYVPGVVST VVPDSAHKLF IGGLPNYLND DQVKELLTSF GPLKAFNLVK DSATGLSKGY AFCEYVDINV TDQAIAGLNG MQLGDKKLLV QRASVGAKNA TLVSPPSTIN QTPVTLQVPG LMSSQVQMGG HPTEVLCLMN MVLPEELLDD EEYEEIVEDV RDECSKYGLV KSIEIPRPVD GVEVPGCGKI FVEFTSVFDC QKAMQGLTGR KFANRVVVTK YCDPDSYHRR DFW
categoryamino acid composition(%)
HydrophobicI(4.2) V(9.9) L(9.0) M(3.3) A(5.7) G(8.7) P(6.6)
AromaticF(5.1) W(0.3) Y(3.0)
HydrophilicR(3.9) K(4.8) E(5.7) D(6.3) Q(5.4) N(4.5) H(1.2) S(5.7) T(4.8) C(1.8)