| BIOISIS ID :: CAN1C4 | ||||
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Description:
Solution-state analysis of Can1 nuclease from T. thermophilus in the presence of cA4 ligand. Can1 was crystallised in presence of Can1. Can1 was purified onsite using size-exclusion chromatography and mixed with cA4 ligand and measured as a batch experiment.
Total datasets deposited with submission: 1
Supporting archive file:
Download: CA4 .zip
Background subtracted and unsubtracted files from batch mode measurement at 4 different ligand concentrations. Buffer and Sample subdirectories denote the respective unsubtracted SAXS measurements.
Data Set:
Can1 Nuclease with cA4 (cyclic tetra-Adenylate)
Description:
Can1 nuclease was purified onsite at the beam line. To measure Can1 in the presence of cA4, an independent, non-SAXS SEC purification of Can1 with manual collection of the main elution peak fraction (~100 μl) . 10.4 mg mL^−1^ protein stock was injected. The peak fraction experiences ~3.2× dilution for a final concentration of 44 μM of purified protein.
Buffer (Matrix):
20 mM Tris pH 7.5 and 150 mM NaCl
Source: synchrotron
Instrument: B21 Diamond Light Source
Deposited files in dataset:
Download CA4-1_average_average.dat
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Real-space Transform: CA4_pr.dat dmax :: 95.0 Loading...
Download CA4_pr.dat
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