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Description:
Experiment was part of a methods book on studies of Calcium binding proteins, specifically Calcium-Binding Proteins of the EF-Hand Superfamily.
Total datasets deposited with submission: 1
Supporting archive file:
Download: CaM.zip
Contains output from Merging along with PDFs of the SEC-SAXS traces and analysis.
Data Set:
SEC-SAXS Calmodulin +/- Calcium
Description:
SEC SAXS was performed using Shodex KW 402.5 column equilibrated with 2 column volumes of buffer. 30 uL of sample was injected and monitored at 40 uL per min with 1 frame per second. Peak of interest was used to produced merged frames.
Buffer (Matrix):
25 mM Na-HEPES pH 7.5 and 150 mM NaCl. Experiments with calcium used 2.5 mM CaCl~2~. Experiments without calcium used 1 mM EGTA.
Source: synchrotron
Instrument: B21 Diamond Light Source
Deposited files in dataset:
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Real-space Transform: apoCAM_average_average_refined_pr.dat dmax :: 64.5 Loading...
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Download plusCalcium_average_average.dat
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Real-space Transform: plusCalcium_average_average_refined_pr.dat dmax :: 65.0 Loading...
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