BIOISIS ID :: HUCAM1

SEC-SAXS of human Calmodulin in presence and absence of Ca 2+

Added: 01 October 2020

Description:

Experiment was part of a methods book on studies of Calcium binding proteins, specifically Calcium-Binding Proteins of the EF-Hand Superfamily.

Total datasets deposited with submission: 1

Supporting archive file:

Download: CaM.zip

Contains output from Merging along with PDFs of the SEC-SAXS traces and analysis.


Data Set:

SEC-SAXS Calmodulin +/- Calcium

Description:

SEC SAXS was performed using Shodex KW 402.5 column equilibrated with 2 column volumes of buffer. 30 uL of sample was injected and monitored at 40 uL per min with 1 frame per second. Peak of interest was used to produced merged frames.

Buffer (Matrix):

25 mM Na-HEPES pH 7.5 and 150 mM NaCl. Experiments with calcium used 2.5 mM CaCl~2~. Experiments without calcium used 1 mM EGTA.

Source: synchrotron

Instrument: B21 Diamond Light Source

Deposited files in dataset:

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Real-space Transform: apoCAM_average_average_refined_pr.dat dmax :: 64.5

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Real-space Transform: plusCalcium_average_average_refined_pr.dat dmax :: 65.0

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